Profile Summary
To create my own niche in the field of Embryology. Assisted reproductive technology and its application that would enhance my inbuilt capabilities, providing an opportunity of intellectual growth in a Practical path and also successful deliver solution to every patient problem.
Educational Background
- Master of science in Assited Reproduction and Clinical Embryology IIARTRC Mysore University -2019.
- Master of Science (M.Sc), sahyadri collage kuvempu university shivamogga 2011 – 2012.
- Bachelor of Science (B.Sc), sahyadri collage kuvempu university shivamogga 2008-2011.
Technical Skills
- IVF,ICSI,PICSI,IMSI,TESA,TESE,PESA,P
- Embryo Biopsy (Day 3 & Day 5)
Instruments Handled
- Electrophoresis unit (AGE & PAGE), Visible Spectrophotometer, High speed refrigerated centrifuge, Column chromatography, ,IVF- Work Station ELITE Series, IVF TECH ,Narishege IX 51, Stereo-Microscope SZT-11 and SZ-
Certifications
- Anderson Diagnostics Certified and validated biopsy skill for blastomere/trophectroderm.
- An “Performing Semen Analysis for the MANTA study,” from Gilead GS-US-418- 4279, TULANE University
- Participated in ACE Pre Congress workshop on Automation, Data Management & Trouble Shooting (2024)
Languages
Work Experience
Vardhan fertility – Chief Clinical Embryologist, Bangalore, 2023 to present
- Aseptic techniques, in vitro fertilization (IVF), Intra Cytoplasmic sperm Injection, Oocytes identification, Oocytes denudation.
- Oocyte grading, Embryo culture (Single step & Sequential) Blast
- Embryo transfer, Documentation and record keeping
- Laboratory ethics, Patient counselling, Cryopreservation and thawing of gametes
- Vitrification of Oocytes and
- Micromanipulator-ICSI- Narishege IX 71
- Laser Assisted Hatching (Zona Thinning & Zona Hatching) –RI.
- Embryo Biopsy(Day 3 and Day 5)
- Complete IVF Lab Quality Control.
- Trained around 04 Andro technicians till
- Trained around 05 Embryologist till
- Embryology Training Academy
Siri Caree - Senior Clinical
Embryologist, Bangalore, August 2019 to september 2023
- Worked as Senior Clinical Embryologist .
- Worked as Teaching faculty at IIARTRC Mysore University
- Trained 2
- SOP training to 100 Embryologist.
Caree Fertility –Junior Embryologist Bangalore –July 2013- August 2019
- Handling Andrology laboratory
- Handling Biochemistry laboratory
Trained around 50 trainee embryologist
Andrology:
- Semen analysis and Functional Test (Sperm count, Motility, Morphology) [WHO & Krugger]
- Sperm wash techniques (Swim up and Density Gradient)
- Semen preparation for IUI
- Cryopreservation and thawing
- DNA Fragmentation Test
Embryology:
- Documentation and record keeping
- Oocyte grading, Aseptic techniques,
- In vitro fertilization (IVF), Intra Cytoplasmic sperm Injection,
- Embryo transfer, Quality Control and Quality Assurance, Vitrification of Oocytes and Embryos
Laboratory Experience
- CELL BIOLOGY, GENETICS AND MLECULAR BIOLOGY – Karyotyping, Vital staining of mitochondria, Chloroplast isolation, Isolation of Bacterial DNA and RNA and analysis by Gel electrophoresis, Isolation of genomic
- GENERAL MICROBIOLOGY – Media preparation, Sterilisation techniques, Bacterial motility, staining techniques (simple, Gram staining, Capsule, Endospore, Fungal, VAM)
- BIOLOGICAL CHEMISTRY – Buffer preparation, TLC (Protein, amino acids), Colorimetric estimation (protein, sugars), PAGE (SDS, Agarose), Gel filtration chromatography (Sepharose, Sephadex),
- IMMUNOLOGY, IMMUNOTECHNOLOGY TECHNIQUES
Research Publications
DAO-9 (2,5-di(4-aryloylaryloxymethyl)-1,3,4-oxadiazole) exhibits p53 induced apoptogenesis through caspase-3 mediated endonuclease activity in murine Biomed Pharmacother (2014), Q1H.D. Gurupadaswamy T.S. Abhishek b , B.T. Prabhakar , http://dx.doi.org/10.1016/j.biopha.2014.07.004
